Fig. 6.

GL7⁺ B cells accumulate within the CNS during JHMV infection. Analysis of GL7 expression in brain derived B cells at day 7, 14, and 21 p.i. by flow cytometry. (A) Representative density plots showing gating strategy of GL7^int (black) and GL7^hi (red) within total CD19+ cells. Numbers in plots indicate relative percentages of GL7^int and GL7^hi populations. (B) Stacked bar graph shows increasing percentages of GL7^int and GL7^hi B cells during viral persistence. (C) Representative histogram comparing GL7 expression intensity on IgD⁺ (red), IgD^int (blue), and IgD⁻ (gray) B cell subsets within CLN and Brain at day 14 p.i. (D) Relative percentages of GL7^int and GL7^hi cells within IgD⁺, IgD^int, or IgD⁻ brain derived CD19⁺ B cells at day 7, 14, and 21 p.i. Data in (B) and (D) are expressed as the mean percentage ± SEM and represent 3–4 independent experiments each comprising 3–6 pooled brains per time point. Significant differences between time points are indicated by ∗ (p ⩽ 0.05) for GL7int, # (p ⩽ 0.05) for GL7hi, and ¶ (p ⩽ 0.05) for total GL7 + cells. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)