Table 1.
EdU labeling of cochlear progenitor cells in two Notch loss-of-function mutants.
|
Total EdU labeled cell types |
||||||||
|---|---|---|---|---|---|---|---|---|
Number of cochleas |
Number of sections |
IHC |
OHC |
BC |
IPC |
PC |
DC |
|
Control |
11 |
314 |
0 (0) |
3 (0.009) |
59 (0.187) |
11 (0.035) |
2 (0.006) |
9 (0.028) |
|
dnMAML1 Mutant |
9 |
239 |
1 (0.004) |
5 (0.02) |
66* (0.276) |
10 (0.041) |
2 (0.008) |
5 (0.020) |
Pofut1 Mutant |
4 |
103 |
0 (0) |
1 (0.019) |
24 (0.233) |
8 (0.077) |
0 (0) |
4 (0.038) |
We administered EdU to pregnant female mice three times a day between E14.5 and E17.5 and collected embryos for analysis at E18.5. The total numbers of dividing cells labeled by EdU for each genotype was normalized by dividing the number of labeled cells by the total number of sections counted. The total number counted for all sections is shown under each cell type and the normalized number per section is shown below in parentheses. A modified Wald test for two-sample proportions was used to determine whether the numbers of dividing cells was significantly different in either mutant group compared with the control groups. Statistical tests were applied to individual hair and supporting cell types (see text). The only group that showed significant differences to control was the number of labeled border cells in dnMAML1 mutants (*p=0.014). IHC: Inner hair cells; OHC: Outer hair cells; BC: Border cells; IPC: inner phalangeal cells; PC: Pillar cells; DC: Deiters’ cells.