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. 2017 Jan 5;7:40126. doi: 10.1038/srep40126

Figure 2. The QS signal-negative plasmid pTiCh can exploit the QS-signals emitted by the QS-signal producing cells.

Figure 2

(a) experimental setup: cells with efficient (S) or impaired (s) signal perception that host the QS signal-negative plasmid (pTiCh), QS-producing cells which emitted none (pTi-traI::Gm), low (pTi-Gm) or high level (pTi-accR::Gm) of QS-signals, and recipient cells C58.00 were mixed at 1:1:1 ratio. (b) After 24-hour incubation, the pTiCh donor and recipient cells and the pTiCh-transconjugants were counted. Conjugation assays were performed in quadruplicate in two independent blocks. Factorial ANOVA revealed a significant effect of pTiCh host cell (F(1,64) = 387.08, p < 0.0001), signal level (F(2,64) = 281.70, p < 0.0001) and their interaction (F(2,64) = 97.13 p < 0.0001). The two experimental blocks did not differ significantly F(1,64) = 0.32, p = 0.72. The r2 for the model = 0.95.