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. 2016 Oct 7;5(12):e1237327. doi: 10.1080/2162402X.2016.1237327

Figure 2.

Figure 2.

IL-15-expanded, Id L-chain-specific human Tcells exhibit delayed cellular senescence. (A) Cell cycle analysis of IL-2- or IL-15-expanded idiotype-specific memory CD8+ T cells after stimulation with OKT3 (1 µg/mL, plate-bound) for 72 h. CD8+ Tcm (CD8+, CD62L+, CD45RA−); CD8+ Tem (CD8+, CD62L−, CD45RA−). (B) Western blot analysis for P53, P21WAF1, and P16INK4a expression of idiotype-specific memory CD8+ T cells expanded by IL-2 or IL-15, before transfer into mice. (C) Intracellular cytokine staining of IL-2- or IL-15-expanded idiotype-specific (P28) memory CD8+ T cells after 5 h of stimulation with PMA (50 ng/mL) and ionomycin (250 ng/mL) in the presence of 10 ug/mL Brefeldin A. (D) Flow cytometry analysis of cell surface markers of IL-2- or IL-15-expanded, L-chain peptide-specific (P28) day 14 T cells, before transfer into mice. (E) Heat map showing the expression of 84 cellular senescence biomarkers by real-time RT-PCR array assays in IL-15- or IL-2-expanded Id L-chain-specific (P28) T cells, before transfer into mice (List of genes is shown in Table 1). MFI: Mean fluorescence intensity. p < 0.05. Tcm = central memory T cells. Tem = effector memory T cells.