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. 2017 Jan 5;7:40050. doi: 10.1038/srep40050

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Copyright © 2017, The Author(s)

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(A) Gene expression of IL1B and IL18 in human PBMCs 30 minutes after LPS or MPLA treatment. (B) Levels of cleaved IL-1β and IL-18 produced by human PBMCs 6 and 24 hours after LPS or MPLA treatment. (C) Expression of NLRP3 (110 kDa) and precursors of Caspase-1 (45 kDa), IL-1B (31 kDa), and IL-18 (24 kDa) in human PBMCs 6 hours after LPS or MPLA treatments. Gels have been cropped for clarity; the bands were confirmed by the comparison with full-length gel images and molecular weight (Supplementary Figure S8). (D) Densitometry analysis of the Western Blot results. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, NS = not significant; n = 4 in each group, and data are representatives of three independent experiments.