Table 1.

Primers in the real-time PCR assay for validating the differential expressions of circRNAs after sequencing.

Target name	Primer name	Sequence (5′ → 3′)	Amplicon size (bp)	Tm (°C)
4A:214775737|215210034	1-Forward 1-Reverse	TTGCCTGTCTTGGGATCTTC GGCAAGGATCCCCCTAAATA	212	60.2 59.8
5B:221655648|222074380	2-Forward 2-Reverse	GTCGATCCTGCTCATGTTGA CCATTGTCGCATCTTGTTTG	215	59.8 60.1
5D:124926662|125373509	3-Forward 3-Reverse	AATAGGCGGAGAACCATTCA CCCCGACTCCTACTTGGTTA	158	59.0 59.4
5D:18963119|19091255	4-Forward 4-Reverse	TGGAGGAGTGGTTCTTCCAC CGTCGAAGGTGACGTACTTG	186	60.1 59.5
6A:201878372|202172929	5-Forward 5-Reverse	ACCTCTAGCCCCACTGTTGA CCAGGAGGCTTGAGAAAGAG	172	59.7 60.1
6D:169739848|170130661	6-Forward 6-Reverse	TTTTCATACCAAGGCAAGCA CTCCCTCATCATCTCCTCCA	201	60.2 60.1
2D:62914891|62916501	7-Forward 7-Reverse	AACTCGGTTTGAGATAAGCTACC CAAATTGAGCAACGCCAAAT	156	58.1 61.0
3B:15806226|15817694	8-Forward 8-Reverse	GCAGGACATGGAAGCTCATC GGCCAGGATCTTCTTCTTCC	185	60.1 60.0
3B:68150153|68152981	9-Forward 9-Reverse	ATTTACCCGAGGACCCACTT GATCCATGGCAACACTTCCT	227	59.7 59.9
IWGSC_CSS_4DS_scaff_2307013:2345|3583	10-Forward 10-Reverse	CCCATTTAAAAACTTGCGTGA GTGCCTTAACAGCCCTCTTG	193	60.0 59.9
7A:11715620|11717078	11-Forward 11-Reverse	TCCAAACATATGGAAGGATGC ACGGCTTCAGTTCAAACACC	192	59.8 60.2
5A:128534161|128535165	12-Forward 12-Reverse	AGGACCTTTGACGCACTGTT TCTCTATTCGCAGTCCATGCT	202	59.8 60.0
7D:12625708|12627116	13-Forward 13-Reverse	TCCAAACATATGGAAGGATGC AAATTCGAGAGGGGTTACCA	199	59.8 58.0
IWGSC_CSS_2BL_scaff_7985665:1994|3344	14-Forward 14-Reverse	AACCTTGTGCCATGGATGTA TCTCTATTCGCAATCCATGCT	175	58.9 59.9
IWGSC_CSS_5AL_scaff_2801994:5461|6705	15-Forward 15-Reverse	TGACATCAGCATCGCAAAAT TCAGCCTCTAACCAGCTTCC	205	60.2 59.6
6B:195302800|195307032	16-Forward 16-Reverse	TTGCGTTCAAGGCTAAAAGG TTTTTCCGGTTGATGAGGAC	198	60.4 59.9
GAPDH	CK-Forward CK-Reverse	ACATTAAGGGTGGTGCCAAG TGGTCATCAAACCCTCAACA	198	59.9 59.9