Figure 1.

Deletion of both alleles of BCY1 in C. albicans. (A) Strategy for BCY1 deletion. Fusion PCR deletion assays were used to delete the two alleles of BCY1 as described in the Methods Section. CdHIS1 and CmLEU2 were used as selective markers for BCY1 deletion. (B) PCR confirmation of BCY1 deletion in the WT strain SN152. The primers used are indicated in (A). Lanes 1 and 3: 3′-flanking checking primers for CdHIS1 integration; lanes 2 and 4: 3′-flanking checking primers for CmLUE2 integration; lanes 5 and 6: BCY1 ORF checking primers. L, DNA ladder.