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Deletion of BCY1 in C. albicans promotes cell death, especially in the bcy1/bcy1 smooth (S) form. Cells of the WT, bcy1/bcy1 filamentous (F), and bcy1/bcy1 smooth (S) forms were incubated in Lee's glucose liquid medium for 48 h with shaking, and then used for DAPI and PI staining assays. The percentages (on PI-images) represent the survival rates using plating assays. DIC, differential interference contrast. Scale bar, 10 μm.
