FIG 1.

Effects of miR-H2 on ICP0 protein and ICP0 mRNA expression in transfected cells. (A) 293T cells were cotransfected with 75 ng of pRS-1 and 16 nM either irrelevant control mimic or miR-H2 mimic. At 30 h posttransfection (hpt), ICP0 and, as a loading control, actin levels were analyzed by Western blotting. (B) 293T cells were cotransfected with pRS-1, 8 nM miR-H2 mimic, and 20 nM either miR-H2 inhibitor (antiH2) or control inhibitor (antiCtrl). At 30 hpt, ICP0 and actin levels were analyzed by Western blotting. (C) Transfection was performed as described in the legend to panel A in triplicate, with ICP0 mRNA levels, normalized to the levels of human GAPDH mRNA, measured by qRT-PCR at 30 hpt. Horizontal bars and error bars represent mean values and standard deviations, respectively.