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. 2017 Jan 3;91(2):e01555-16. doi: 10.1128/JVI.01555-16

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FIG 2 — HSP70 copurifies with CLSV. (i) Western blot of CLSV virion preparations (extracted by differential centrifugation as described for CNV) probed with an HSP70 antibody. CLSV particles (10,000, 20,000, and 40,000 ng, as indicated) were electrophoresed under denaturing conditions and blotted to PVDF membranes prior to incubating with antibody. Dilutions of N. benthamiana Hsc70-2/His₇ (1.25, 2.5, and 5.0 ng) probed with HSP70 antibody were used to estimate the amount of HSP70 present in CLSV virion preparations. (ii) Summary of amounts of HSP70 present in virion preparations as determined by densitometry using Image Lab software version 5.1 (Bio-Rad).