TABLE 1.

Primers and probes for seven porcine reference genes, eleven cytokines, and six PRV genes were either selected from previous studies or designed using the PrimerQuest tool

Gene or cytokine	Description	Primer or probe sequence (5′–3′)			Reference or source
		Forward primer	Reverse primer	Probe
Reference genes
ACTB	β-Actin	AGCGCAAGTACTCCGTGTG	CGGACTCGTCGTACTCCTGCTT	TCGCTGTCCACCTTCCAGCAGATGT	29
B2M	β2-Microglobulin	AAACGGAAAGCCAAATTACC	ATCCACAGCGTTAGGAGTGA	AGAAGATGAACGCGGAGCAGTCAG	30 (primers); PrimerQuest (probe)
GADPH	Glyceraldehyde-3-phosphate dehydrogenase	ACATGGCCTCCAAGGAGTAAGA	GATCGAGTTGGGGCTGTGACT	CCACCAACCCCAGCAAGAGCACGC	29
HPRT1	Hypoxanthine phosphoribosyltransferase 1	GTGATAGATCCATTCCTATGACTGTAGA	TGAGAGATCATCTCCACCAATTACTT	ATCGCCCGTTGACTGGTCATTACAGTAGCT	28
PPIA	Peptylpropyl isomerase A of cyclophilin A	TGCTTTCACAGAATAATTCCAGGATTTA	GACTTGCCACCAGTGCCATTA	TGCCAGGGTGGTGACTTCACACGCC	28
SDHA	Succinate dehydrogenase complex, subunit A, flavoprotein	GAACCGAAGATGGCAAGA	CAGGAGATCCAAGGCAAA	CTGCACACGTTGTACGGAAGGTCT	Primers (30); PrimerQuest (probe)
UBC	Ubiquitin C	GCGCACCCTGTCTGACTACA	AGATCTGCATCCCACCTCTGA	AGTCCACCCTGCACCTGGTCCTCC	31
Cytokines
IFN-α	Interferon alpha	TGGTGCATGAGATGCTCCA	GCCGAGCCCTCTGTGCT	CAGACCTTCCAGCTCT	29
IFN-β	Interferon beta	AGCAGATCTTCGGCATTCTC	GTCATCCATCTGCCCATCAA	TAGCACTGGCTGGAATGAAACCGT	PrimerQuest
IFN-γ	Interferon gamma	CGATCCTAAAGGACTATTTTAATGCAA	TTTTGTCACTCTCCTCTTTCCAAT	ACCTCAGATGTACCTAATGGTGGACCTCTT	28
TNF-α	Tumor necrosis factor alpha	AACCTCAGATAAGCCCGTCG	ACCACCAGCTGGTTGTCTTT	CCAATGCCCTCCTGGCCAACG	29
IL-1α	Interleukin-1α	GTGCTCAAAACGAAGACGAACC	CATATTGCCATGCTTTTCCCAGAA	TGCTGAAGGAGCTGCCTGAGACACCC	28
IL-1β	Interleukin-1β	GTGCTGGCTGGCCCACA	GAACACCACTTCTCTCTTCA	CTCTCCACCTCCTCAAAGGG	29
IL-2	Interleukin-2	TGCTGATCTCTCCAGGATGC	CCTCCAGAGCTTTGAGTTCTTCTACTA	AAGCAGGCTACAGAATTGAAACACCTT	29
IL-4	Interleukin-4	GTCTGCTTACTGGCATGTACCA	GCTCCATGCACGAGTTCTTTCT	CCACGGACACAAGTGCGACATCACCTTAC	29
IL-6	Interleukin-6	CTGGCAGAAAACAACCTGAACC	TGATTCTCATCAAGCAGGTCTCC	TGGCAGAAAAAGACGGATGC	29
IL-8	Interleukin-8	AAGCTTGTCAATGGAAAAGAG	CTGTTGTTGTTGCTTCTCAG	TCTGCCTGGACCCCAAGGAAAAGT	29
IL-10	Interleukin-10	CGGCGCTGTCATCAATTTCTG	CCCCTCTCTTGGAGCTTGCTA	AGGCACTCTTCACCTCCTCCACGGC	28
Viral genes
IE180	Immediate early 180	GAAGCGGGCGAGAGAAAT	GGGATTTCGCGAACTT	TCGCGAGGACCATTTGCATGC	PrimerQuest
EP0	Early protein 0	TTTGACATATCGGCGCAGT	AGAGTGTGCCTCGGACT	ATGAGCCACCAGGGTGTGAACTAT	PrimerQuest
gB	Glycoprotein B	ACCACCGGCGCTACTTTAAG	CCTCCAGCAGCGTCAGGTT	ACGATCAGCACGCGGGTGACC	PrimerQuest
gC	Glycoprotein C	CTTCGAGGTCCGCTTCTAC	TTGGCGGAGCTGAAGAG	CCGAGTACTTTGACGAGCCC	PrimerQuest
gE	Glycoprotein E	GCTTCCACTCGCAGCTCTTCT	GATGCAGGGCTCGTACACGTA	CCGCGCGTGGTCTCGGACA	PrimerQuest
LAT intron	Intron of latency-associated transcript	CTTCTTCACACGGAGGACAC	AAGCCACGCTGATACTCTTG	ATGGGCAGCAGATGGTACGGTC	PrimerQuest