FIG 2.

Direct interaction between pUL7 and pUL51. GST-tagged pUL7, His₆-tagged full-length pUL51 with the cysteine at residue 9 mutated to serine to prevent aberrant disulfide bond formation (1–244 C9S), and truncated pUL51 (residues 29 to 170) proteins were expressed in E. coli and purified using affinity and size exclusion chromatography. Purified His₆-tagged N1 protein from vaccinia virus was included as a negative control. GST-pUL7 or GST alone was captured on glutathione-magnetic beads and incubated with soluble pUL51 or N1, the beads were washed, and associated proteins were eluted for separation by SDS-PAGE. The black arrowheads denote full-length and truncated pUL51, while the white arrowhead denotes N1.