TABLE 2.
Oligonucleotide primers
| Name | Sequence (5′–3′) | Details |
|---|---|---|
| COL450 | CCGCACCTCGCCCTGGCCCATCTCGAGCAGCTCCGTGTGTTTTGGGTCAAGTGAGCAAGGGCGAGGAG | Forward primer for inserting in-frame EYFP(A206K) at C terminus of pUL51 |
| COL451 | CTCCATCCCACAAACACAAAACACACGGGTTGGATGAAAACACGCATTTACTTGTACAGCTCGTCCATG | Reverse primer for inserting in-frame EYFP(A206K) at C terminus of pUL51 |
| COL456 | TTTCGGAGACCCCAAAACGACAGACGTCGTCGCTGTTTTATCAGTTTTGTGTGAGCAAGGGCGAGGAG | Forward primer for inserting in-frame EYFP(A206K) or mCherry at C terminus of pUL7 |
| COL457 | ATCCGTCGGGAGGCCACAGAAACAAAACCGGGTTTATTTCCTAAAATTCACTTGTACAGCTCGTCCATG | Reverse primer for inserting in-frame EYFP(A206K) or mCherry at C terminus of pUL7 |
| COL496 | CGCCGCGACGGCCGACGATGAGGGGTCGGCCGCCACCATCTAGTAATGAATTCTCGCCGGGGACCGCAGCCTGAGGATGACGACGATAAGTAGGG | Forward primer for replacing codons 16–18 of UL7 with three stop codons |
| COL497 | ATCGCCTCGGCCGCCTCGACCAGGCTGCGGTCCCCGGCGAGAATTCATTACTAGATGGTGGCGGCCGACCCCTCAACCAATTAACCAATTCTGATTAG | Reverse primer for replacing codons 16–18 of UL7 with three stop codons |
| COL498 | TATATGTGGCTGGGGAGCGCGCCCCGAGGAACAATATGAGTAGTGATAAGGATCCGTTCCGCCCTCGGAGGCGGAAGGATGACGACGATAAGTAGGG | Forward primer for replacing codons 21–23 of UL51 with three stop codons |
| COL499 | GGGCCTCCTGCAGCCGCGGCTCCGCCTCCGAGGGCGGAACGGATCCTTATCACTACTCATATTGTTCCTCGGGGCCAACCAATTAACCAATTCTGATTAG | Reverse primer for replacing codons 21–23 of UL51 with three stop codons |
| COL523 | GGGGTTTCTTCTCGGTGTTTGTGTTGTATCGTGCTTGGCGTAGTGATAAGCTTCGTCCTGGAGACGGGTGAGTAGGATGACGACGATAAGTAGGG | Forward primer for replacing codons 21–23 of US8 with three stop codons |
| COL524 | AACGAAACGTCCTCGCCGACACTCACCCGTCTCCAGGACGAAGCTTATCACTACGCCAAGCACGATACAACACCAACCAATTAACCAATTCTGATTAG | Reverse primer for replacing codons 21–23 of US8 with three stop codons |