FIG 1.

Stabilization of HLA-A*02:01 molecules on the surface of T2 cells by VP13/14 peptide epitopes. T2 cells (3 × 10⁵) were incubated with serial dilutions of the indicated VP13/14 peptide, as described in Materials and Methods. Cells were then stained with an FITC-conjugated anti-HLA-A2 MAb (BB7.2). The graph represents the increase in the expression of HLA-A2 molecules on the surface of T2 cells triggered by various concentrations of VP13/14 peptides, and the data represent the percent MFI increase, which was calculated as follows: [(MFI with the given peptide − MFI without peptide)/(MFI without peptide)] × 100. Solid lines, peptides that bind to HLA-A02:01 molecules with a moderate to high affinity; broken lines, peptides that bind to HLA-A02:01 molecules with a low affinity. Error bars show SDs obtained from 3 independent experiments.