FIG 3.

More VP13/14 epitope-specific CD44^high CD62L^low CD8⁺ T_EM cells are detected in ASYMP individuals than in SYMP individuals. The T_EM- and T_CM-cell phenotypes of CD8⁺ T cells specific to VP13/14_286–294, VP13/14_504–512, and VP13/14_544–552 epitopes among PBMCs of 10 HLA-A*02:01-positive, HSV-1-seropositive SYMP individuals and 10 HLA-A*02:01-positive, HSV-1-seropositive ASYMP individuals were analyzed by FACS. (A, C, and E) Representative FACS data on the frequencies of CD44^high CD62L^low CD8⁺ T_EM cells and CD44^high CD62L^high CD8⁺ T_CM cells in PBMCs from one SYMP individual and one ASYMP individual. (B, D, and F) Average frequencies of VP13/14-specific T_EM or T_CM CD8⁺ T cells from 10 SYMP and 10 ASYMP individuals. (G, I, and K) Representative FACS data on the frequencies of CD8⁺ CD45RA⁻ CCR7⁻ T_EM cells and CD8⁺ CD45RA⁻ CCR7⁺ T_CM cells in one ASYMP individual and one SYMP individual. (H, J, and L) Average frequencies of VP13/14-specific T_EM and T_CM CD8⁺ cells from 10 ASYMP and 10 SYMP individuals. The results are representative of those from 2 independent experiments. The indicated P values, calculated using an unpaired t test, show statistically significant differences between ASYMP and SYMP individuals.