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Y102 mutants associate with Gps2 and Tax1BP1. (A) HEK293TT cells were transfected with 1 μg each mRFP-GFP (−), WT BPV-1 E2 and mutants (YF and YE) plus E2R, and hemagglutinin-tagged Gps2 (HA-Gps2) (pHA:AMF1) or pCI. Western blots were probed for HA-Gps2 and BPV-1 E2 by using mouse monoclonal anti-HA antibodies HA-7 and B201, respectively. (B) Samples were prepared as described above for panel A by using p3XFLAG-CMV-7.1 Tax1BP1. Western blots were probed with FLAG-M2 and B201.
