FIG 6.

Y102 mutants localize to nuclei. CV-1 and A3 (positive control for E2 expression [+]) cells were cultured on 18-mm glass coverslips. CV-1 cells were transfected with 100 ng pCG-E1 and 100 ng pEGFP-C1 (eGFP [−]), WT E2, the Y102F or Y102E mutant, or E2R. Forty-eight hours after CV-1 cell transfection, cells were fixed and permeabilized, followed by incubation with B201. Cells were reacted with Alexa Fluor 594 anti-mouse antibody, washed, and mounted onto glass slides by using ProLong Gold with DAPI. Green, eGFP; red, BPV-1 E2; blue, DNA. Bar, 10 μm. All images were taken in all three channels (green, red, and blue) at a ×1,000 magnification.