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C127 cell lines do not maintain Y102F and Y102E episomes. (A) Total DNA from transformed C127 cells containing Y102E, Y102F, or wild-type BPV genomes was subjected to Southern blotting using a BPV LCR-specific probe. The number below each genome represents an individual clonal cell line. (B) A 677-bp PCR amplicon of the LCR region of the same cell lines represented in panel A demonstrates that each clonal population contains BPV DNA.
