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. 2017 Jan 3;91(2):e01859-16. doi: 10.1128/JVI.01859-16

FIG 1.

FIG 1

Antibody PL-2 binds with high specificity and affinity to Spike2. (A) Crystal structure of Spike2, with one half of the dimer in gray and the other half in red. The gray side has labeled β-sheets and α-helices. (B) Structural comparison of Spike1 (green), Spike2 (red), and Spike8 (cyan). (C) ELISA results showing that MAb PL-2 binds to Spike2 but not Spike1 or Spike8. (D) ELISA results showing that scFv PL-2 binds to Spike2 but not Spike1 or Spike8. (E) Background-subtracted SPR traces (orange) and curve fit with a 1:1 binding model (black). Sequential injections of increasing concentrations of Fab PL-2 were tested with immobilized Spike2. kon = 3.45 × 105 M−1 s−1, koff = 6.02 × 10−4 s−1, and Chi2 (RU2) = 1.36. (F) Background-subtracted SPR traces (brown) and curve fit (black). Sequential injections of increasing concentrations of scFv PL-2 were tested with immobilized Spike2. kon = 6.55 × 105 M−1 s−1, koff = 1.23 × 10−3 s−1, Chi2 (RU2) = 2.06.