FIG 6.

Mitochondrial localization of UL16 is triggered by substitutions in its C-terminal domain. The indicated derivatives of full-length UL16 and its N- and C-terminal domains were expressed individually in Vero cells and detected by GFP fluorescence or by staining with antibodies specific for the myc or HA tag (green). Mitochondria were stained with MitoTracker Red. Confocal images of the various full-length constructs are shown in row 1 of panel A (wild type) and all rows of panel B (two different cells for each mutant). All the remaining images are of an NT or CT construct of UL16. Two different cells expressing wild-type CT are shown in rows 3 and 4 of panel A, with the inset showing an enlarged region to highlight its occasional mitochondrial localization. Mutants of CT are shown in the bottom three rows of panel A.