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. 2017 Jan 5;7:39900. doi: 10.1038/srep39900

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This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Representative HPLC-ESI-MS/MS chromatograms before and BME treatment of 10-NO₂-OA metabolites in (a) human and (b) rat urine. Excess BME treatment leads to the complete consumption of electrophilic 10-NO₂-OA derivatives as evidenced by the disappearance of precursor peaks from their original retention times (lower panels).