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. 2017 Jan 5;7:40088. doi: 10.1038/srep40088

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Copyright © 2017, The Author(s)

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ALP staining (A) and ALP activity (C) test were performed after 1 and 2 weeks of osteogenic induction, respectively. Alizarin Red staining (A) and quantitative analysis (B) were performed after 4 weeks of osteogenic induction. The expression levels of Runx2, ALP, Col-I and periostin investigated by qRT-PCR after PDLSCs indirectly co-cultured with JBMSCs/IBMSCs on Ti. The expression levels were normalized to β-actin with osteogenic induction for 1 (D) and 2 weeks (E). The data are shown as the mean ± SD; *P < 0.05, n = 3.