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. 2017 Jan 5;7:40041. doi: 10.1038/srep40041

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Confocal image showing GCaMP6s (green) in layer II/III neurons and tdTomato (red) expression in PV interneurons. (b,b₁) GCaMP6s-expressing neurons. Cells are numbered in (b₁). Depth: 190 μm. (c) Simultaneous scanless imaging and single-photon stimulation in vivo. Response to three light stimuli is shown. Traces are expressed as (F(t) − bg(t))/bg(t). (d) Pair-wise correlation in the absence (light off, top) and presence (light on, bottom) of wide-field stimulation for the experiment displayed in (c).