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. 2017 Jan 5;7:40089. doi: 10.1038/srep40089

Figure 1. HBx is critical in HBV promoting miR-181a expression in hepatocyte.

Figure 1

(A) miRNA expression heat map depicting differentially expressed miRNAs (n = 3, p < 0.05) in HepG2 and HepG2.2.15 cells. (B) A total of 62 miRNAs were up-regulated in HepG2.2.15 compared to HepG2 cells (n = 3, p < 0.05) (the top elevated 25 microRNAs were listed). (C) qRT-PCR analysis of miR-181a in HepG2 and HepG2.2.15 cells. (D) qRT-PCR analysis of miR-181a in HepG2 cells transfected with HBV1.3 vector and with the corresponding control (pCDNA3.0). (E) qRT-PCR assay for miR-181a in HBs-, HBc-, HBp-, or HBx-expressing cells and the corresponding controls. The data represent the mean ± SD. (F) Dual-luciferase assay for the miR-181a promoter activity of HBV or HBx-expressing cells relative to the corresponding control, versus the blank group (n = 3, *p < 0.05, ***p < 0.001).