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. 2017 Jan 5;7:40089. doi: 10.1038/srep40089

Figure 3. MiR-181a directly inhibits the expression of PTEN by binding 3′UTR of PTEN mRNA.

Figure 3

(A) Dual-luciferase assay of PTEN-3′UTR activity in HepG2 cells transfected with miR-181a at different doses (pRNAT-miR-181a vector 1 μg, 2 μg, 4 μg, respectively) and the corresponding controls (pRNAT vector 1 μg, 2 μg, 4 μg, respectively), versus the blank group. (B) Western blotting analysis for PTEN in HepG2 cells transfected with miR-181a at different doses (pRNAT-miR-181a vector 1 μg, 2 μg, 4 μg, respectively) and the corresponding controls (pRNAT vector 1 μg, 2 μg, 4 μg, respectively), versus the blank group. (C) Western blotting analysis for PTEN in HepG2 cells transfected with miR-181a inhibitor (miR-181In10, miR-181In 20) and the corresponding controls (NC10, NC20) at different doses (10 μl, 20 μl, respectively), versus the blank group (n = 3, **p < 0.01, ***p < 0.001).