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. 2017 Jan 5;7:40089. doi: 10.1038/srep40089

Figure 4. MiR-181a inhibitor abolished the inhibitory effect of HBx on PTEN 3′UTR and PTEN protein expression.

Figure 4

(A) Dual-luciferase assay of PTEN-3′UTR activity in HepG2 cells transfected with pHBx and the corresponding control (pcDNA3.0), cotransfected with pHBx and miR-181a inhibitor (HBx + miR-181In) and the corresponding control (HBx + NC), versus the blank group (p < 0.001). (B) Western blotting analysis for PTEN in HepG2 cells transfected with pHBx and the corresponding control (pcDNA3.0), pHBx cotransfected with miR-181a inhibitor (HBx + miR-181In), pHBx cotransfected with the corresponding control of miR-181a inhibitor (HBx + NC), versus the blank group. (C) Western blotting analysis for PTEN in HepG2.2.15 cells and the cells transfected with miR-181a inhibitor (miR-181In1, miR-181In2, miR-181In3) and the corresponding controls (NC1, NC2, NC3) at different doses (0.125 pM, 0.25 pM, 0.5 pM, respectively), versus the blank group (n = 3, ***p < 0.001).