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. 2017 Jan 5;7:40089. doi: 10.1038/srep40089

Figure 6. HBx promotes cell proliferation activity via up-regulating miR-181a and down-regulating PTEN.

Figure 6

The clone formation test of HepG2 cells transfected with miR-181a and the corresponding controls, cotransfected with miR-181a and the mutation vector of PTEN, cotransfected with miR-181a and PTEN (A pRNAT, B miR-181a, C miR-181a + PTEN-mut, D miR-181a + PTEN). The clone formation test of HepG2 cells transfected with pHBx and the corresponding controls, cotransfected with pHBx and miR-181a inhibitor (miR-181In) and the corresponding controls, cotransfected with pHBx and the mutation vector of PTEN, cotransfected with pHBx and PTEN (E pCDNA3.0, F HBx, G HBx + NC, H HBx + miR-181In, I HBx + PTEN-mut, J HBx + PTEN).