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. 2016 Oct 3;114(2):384–396. doi: 10.1002/bit.26086

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© 2016 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Example of a plasmid‐to‐genome junction and SD‐MMEJ mechanism requiring a templated insertion. (A) A scheme showing the mechanism of plasmid (dark green) joining with the genome (blue). Another fragment of the plasmid (light green) serves as an adaptor providing the microhomologies required for joining and becomes incorporated into the junction as a templated insert. (B) Sequences of plasmid and genome fragments shown in panel A. The integration site and junction sequence used in this example is taken from Supplementary Table SIV (clone BS01, integration site #1, right junction). P1/P2 and P3/P4, primer repeats; mh1/mh2 and mh3/mh4, microhomology repeats.