Fig 2. TGF-β1 and β3 inhibit the expression of mature IgG and IgA transcripts and AICDA.

Naïve B cells were cultured under IL-21, IL-4, and sCD40L stimulation for 4 days, and the expression of mature IgG1, IgG2, IgG3, IgA1, and IgA2 transcripts, as well as AICDA, were assessed by quantitative PCR. Expression relative to GAPDH is shown. TGF-β treated cells were compared with TGF-β untreated cells using one way ANOVA, Dunnett test (n = 3). Results are representative of two similar experiments.