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. 2017 Jan 4;12(1):e0169646. doi: 10.1371/journal.pone.0169646

Fig 6. TGF-β1 and β3 inhibit TFs essential for B cell differentiation into ASCs.

Fig 6

(a-d) B cells were cultured under IL-21 and CD40L stimulation for 3 days (a, b) or 5 days (c, d), and the expression of the indicated genes was assessed by quantitative PCR. Expression relative to GAPDH is shown. TGF-β treated cells were compared with TGF-β untreated cells using one way ANOVA, Dunnett test (n = 5). Results are representative of two (b), three (d), or four (a,c) independent experiments. (e) B cells were cultured under IL-21, CD40L, and CpG-ODN2006 stimulation for 4 days with or without TGF-β. Expression of Blimp-1 on CD19+ B cells was assessed by intracellular staining. TGF-β treated cells were compared with TGF-β untreated cells using one way ANOVA, Dunnett test (n = 3). Results are representative of three similar experiments.