Figure 1.

Thyroid hormone receptor β agonist GC-1 induces hepatocyte proliferation in wild-type mice. (A) Representative photomicrographs (100×) of immunohistochemistry for BrdU and PCNA showed increased proliferation in GC-1-treated mouse livers compared with DMSO controls. Nuclear cyclin D1 was also increased in GC-1-treated mice. (B) Western blot of whole-liver lysates showed increased cyclin D1 and pSer675-β-catenin in GC-1-treated mice compared with DMSO control. (C) Bar graphs shows that the difference in ALT and bilirubin (BR) levels between the experimental groups and control animals was not significant (NS), and all values were within normal limits. (D) Comparable normal hepatic histology in 8-day DMSO and GC-1-injected control mice (100×).