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. Author manuscript; available in PMC: 2017 Jul 15.
Published in final edited form as: Arch Biochem Biophys. 2016 Mar 2;602:61–68. doi: 10.1016/j.abb.2016.02.011

Figure 3. Negative-stain TEM analysis of bulk fragmentation methods for rod-like 2,3 – HPCD microcrystals.

Figure 3

A,B. TEM images of H200Q (A) or WT (B) crystals after fractionation with centrifugation.

C,D. TEM images of H200Q (C) or WT (D) crystals after fractionation with vortexing.

E,F. TEM images of H200Q (E) or WT (F) crystals after fractionation with sonication.

Lattice quality was evaluated when single crystals were observed and median values of 3rd order Bragg spots (not shown) for all samples across the varying fragmentation protocols. (Scale bars – A-F: 1 μm)