Table 1.
Comparison of physical properties and processing characteristics for the HPCD samples used for SFX data collection
| (H200Q HPCD) | (WT HPCD) | |
|---|---|---|
| Density (crystals/ml)a | 1.2 × 108 (±0.1) | 1.53 × 108 (±0.07) |
| Size distributionb | ||
| a ~ b (μm) | 2.5 (±0.5) | 2.6 (±0.6) |
| c (μm) | 11.7 (±5.6) | 10.4 (±4.1) |
| Number of Bragg Spotsc | 4th order | 3rd order |
| Indexing efficiency (%)d | 12.2 | 1.7 |
| 2nd lattice hits (%)e | 16.1 | 8.4 |
a
Values for crystal density quantification using nominal 15x magnification (n=12) as described in Experimental Procedure.
b
Size distribution for fragmented microcrystal samples was determined manually from UV imaging data at nominal 15x magnification (n=15). Mean values for short (a, b) and long (c) dimensions for rod-like morphology.
c
Bragg Spot order calculated from FFT transforms of observable lattices (median derived from n=8).
d
The percentage of collected images that contained indexed patterns (single hits and 2nd lattices).
e
Percentage of double hits in the total number of indexed patterns.