Figure 1.

Characterization of ZIKV strain GZ01 in 129Sv/Ev mice. Group of 4-week-old 129Sv/Ev mice were infected ip with 10⁵ PFU of ZIKV strains GZ01/2016. The animals were observed daily for weight change (A). At days 1, 2, 3, 5 and 7 pi, blood was collected and analyzed by RT-qPCR for viremia (B). At days 1, 2 and 3 pi, 2 anesthetized mice were harvested, weighed, homogenized, and analyzed by RT-qPCR (C). Histopathology findings in indicated tissue of ZIKV-infected mice at day 1 pi. Therapeutic effects of human convalescent phase serum in ZIKV-infected 129Sv/Ev mice. PBS treatment was set as mock group. Viremia at days 1, 2, and 3 pi were determined by RT-qPCR (E). Viral RNA loads in spleen and testis at day 3 pi were determined by RT-qPCR (F). Viremia in 8-week-old 129Sv/Ev mice infected with GZ01 (G). Viral loads in serum or tissue are expressed as RNA copies per milliliter or RNA copies per gram, respectively. Results are shown as the mean ± standard error of the mean and statistical analysis was performed using the unpaired, 2-tailed t-test.