Figure 1.

Schematic of human and zebrafish mfrp loci. (A) Membrane frizzled-related protein contains a transmembrane domain, two cubulin domains, two LDLA domains, and a Frizzled domain. Zebrafish frameshift mutants were made by separately targeting the two cubulin domains using CRISPR guide RNAs. (B, C) Human and zebrafish mfrp chromosomal regions are shown with c1qtnf5. Human mfrp is immediately adjacent to c1qtnf5 but is separated by more than 22 Mbp in zebrafish. Scale bar: 1 kb. (D, D') Alignments show DNA and protein sequences, respectively, of wild-type and mutant alleles of zebrafish mfrp generated using CRISPR 1. (E, E') Alignments show the DNA and protein sequences of the wild-type and mutant alleles generated using CRISPR 2. Red highlighting indicates edited bases.