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Zebrafish mfrp mutants do not show significant changes in mfrp or c1qtnf5 mRNA levels. (A) Quantitative RT-PCR on cDNA synthesized from eyes of mfrp mutants showed no significant change in levels of mfrp mRNA compared with wild-type controls assessed by t-test. (B) Similarly, no significant change in levels of c1qtnf5 mRNA compared with wild-type controls. In both experiments, two primer sets were independently used.
