Fig 1. Identification of AtrR-family genes in A. oryzae and A. nidulans.

(A) Growth of A. oryzae strains overexpressing the candidate genes on medium plate in the presence of clotrimazole. 10² conidia of the strains were inoculated on CD plate (1% maltose) containing 0.5 μg/ml clotrimazole and incubate at 30°C for 7 days. (B) Growth of the AoatrR deletion strain on medium plate in the presence of miconazole. 10² conidia of A. oryzae LigD (wild type) and the AoatrR deletion mutant (ΔAoatrR) were inoculated on CD plate (1% glucose) containing 0.01 μg/ml miconazole (MCZ) and incubate at 30°C for 5 days. (C) Growth of the AnatrR deletion strain on medium plate in the presence of MCZ. 10² conidia of A. nidulans KU70 (wild type) and the AnatrR deletion mutant (ΔAnatrR) were inoculated on CD plate (1% glucose) containing 0.005 μg/ml miconazole and incubate at 37°C for 5 days. (D) Phylogenetic tree of fungal AtrR ortholog proteins. The protein sequences were retrieved from NCBI database, and the sequence IDs were shown in brackets. The sequences were aligned by Clustal W program and the distances were calculated. The phylogenetic tree was drawn with an NJplot program.