Fig 3. Expression analysis in atrR and srbA deletion mutants.

(A) Expression levels of the cyp51A and cyp51B as well as the atrR and srbA genes were determined by real-time RT-PCR method. The strains were cultivated in GMM for 24 h. The expression levels of gene of interest were normalized with that of actin gene. Relative expression ratios against Af293 (WT) are shown. Error bars represent the standard deviations based on three independent replicates. (B) MCZ susceptibility test for the Af293, ΔatrR, and ΔsrbA strains carrying a cyp51A gene fused with a thiA promoter. MCZ susceptibility was examined as described above. When thiamine was present, expression of the functional cyp51A mRNA driven by thiA promoter was repressed. The plates were incubated for 48 h before photographed. (C) Expression levels of cyp51A were determined by real-time RT-PCR in the mutant strains of ΔatrR, ΔsrbA, ΔatrR ΔsrbA of Afs35-background. The strains were cultivated in YGMM for 18 h.