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. 2017 Jan 4;13(1):e1006096. doi: 10.1371/journal.ppat.1006096

Fig 4. AtrR and SrbA regulate genes associated with the ergosterol biosynthesis pathway.

Fig 4

(A) The AtrR- and SrbA-dependent genes determined by RNA-sequencing analysis. Numbers of gene with more than 4-fold or less than 1/4-fold change in expression level compared to Af293 are shown. The expression level of nine genes in ΔatrR and ΔsrbA strains was more than 4-fold of Af293, and the expression level of eighteen genes in ΔatrR and ΔsrbA strains was less than 1/4-fold of Af293. (B) Heat map of expression of the erg genes. The levels of expression ratio were shown. (C) Expression levels of erg3B, erg24A, erg25A, and cyp51A genes in response to MCZ addition were determined by real-time RT PCR method. The strains were cultivated in GMM for 24 h followed by 2 h culture with MCZ (final concentration, 2 μg/mL) or without MCZ (control). The expression levels of gene of interest were normalized with that of actin gene. Relative expression ratios against Af293 (WT without MCZ) are shown. Error bars represent the standard deviations based on three independent replicates.