Fig 6. AtrR is involved in A. fumigatus virulence.
(A) Mouse infection test with the Af293-background strains. Outbred ICR mice (n = 12) were immune-compromised by intraperitoneally injected cyclophosphamide (200 mg/kg) at Day -4, -2, 1, 3, 5, and 7. Mice were infected intratracheally with 2.5×107 conidia in a volume of 25 μL of each strain (Af293, ΔatrR, and Co-atrR). Statistical significance was examined by log-rank test. P value for comparison between ΔatrR and Af293 was lower than 0.01. (B) Mouse infection test with the Afs35-background strains. Outbred ICR mice (n = 9 to 11) were immune-compromised by intraperitoneally injected cyclophosphamide (150 mg/kg) at Day -4, -1, 3, 6, 9, and 12. Cortisone acetate was also administrated subcutaneously at a concentration of 200 mg per kg of body weight on day -1. Mice were infected intratracheally with 3×105 conidia in a volume of 30 μL of each strain (Afs35, ΔatrR, ΔsrbA, and ΔatrR ΔsrbA) on Day 0.