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. 2017 Jan 4;13(1):e1006096. doi: 10.1371/journal.ppat.1006096

Fig 7. FLCZ- and MCZ-responsive and AtrR-dependent genes determined by RNA-sequencing analysis.

Fig 7

(A) The ΔatrR and Af293 strains were cultivated in PDB for 20 h followed by FLCZ (final concentration, 64 μg/mL), MCZ (2 μg/mL), or DMSO (as a control) treatment for 2 h. Number of gene with less than 1/3-fold change in expression level in ΔatrR (DMSO) compared to Af293 (DMSO) was shown. Numbers of gene with more than 3-fold upregulation upon FLCZ or MCZ treatment (2 h) in Af293 were shown. Expression of six genes was induced upon FLCZ and MCZ treatment and these genes were dependent on AtrR, and expression of thirteen genes was induced upon FLCZ and MCZ treatment and these genes were independent on AtrR. (B) Expression level of atrR and srbA genes in response to MCZ addition was determined by real-time RT PCR method. The Af293 strain was cultivated in GMM for 24 h followed by 2 h culture with MCZ (final concentration, 2 μg/mL) or without MCZ (control). The expression levels of gene of interest were normalized with that of actin gene. Relative expression ratios against control (Af293 without MCZ) are shown. Error bars represent the standard deviations based on three independent replicates.