Fig 8. Expression levels of the cdr1B gene in response to azoles were determined by real-time RT PCR method.

(A) AtrR is involved in regulating cdr1B expression. Af293, ΔatrR, and ΔsrbA strains were cultivated in GMM for 24 h followed by 2 h culture with or without MCZ (final concentration, 2 μg/mL). (B) Af293 and ΔatrR strains were cultivated in GMM for 24 h followed by 2h culture with ITCZ, MCZ, or propiconazole (PRCZ) (final concentrations, 2 μg/mL). The expression level of cdr1B was normalized with that of actin gene. Relative expression ratios against Af293 (WT without drug) are shown. Error bars represent the standard deviations based on three independent replicates.