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. 2017 Jan 4;13(1):e1006096. doi: 10.1371/journal.ppat.1006096

Fig 10. The clinical azole resistant strain with an atrR gene deleted shows azole hyper-susceptibility.

Fig 10

(A) Drug susceptibility test by paper-disc diffusion assay. A paper-disc was placed on center of the GMM plate, and 10 μl of drug solution indicated was dropped on it (FLCZ: 10 mg/mL; MCZ: 10 mg/mL; ITCZ: 10 mg/mL). An azole resistant strain IFM 61567 and the independently obtained atrR deleted strains (ΔatrR No. 2–4) were streaked outward from the center. The plates were incubated for 48 h before photographed. The IFM 61567 fully grew along the streaked line regardless of the presence of FLCZ and ITCZ, whereas the ΔatrR mutants showed growth inhibition. (B) Expression levels of cdr1B, cyp51A, and cyp51B genes in IFM 61567 and the atrR-deleted strain (ΔatrR No.2) were determined by real-time RT PCR method. IFM 61567 and the ΔatrR were cultivated in YPG for 18 h followed by 1 h culture with or without FLCZ, MCZ, and ITCZ (final concentrations, 2 μg/mL). The expression levels of genes of interest were normalized with that of actin gene. Relative expression ratios against IFM 61567 without drug (control) are shown. Error bars represent the standard deviations based on three independent replicates.