Figure 4.

CUC3::PHOT1–GFP restores leaf positioning and leaf expansion in the phot1 phot2 double mutant.

Leaf positioning and leaf expansion of wild‐type (WT), phot1 phot2 (p1p2) mutant and three independent lines expressing CUC3::PHOT1–GFP (CUC3::P1–GFP lines 1, 11 and 18).

(a) Seedlings were grown on soil under white light at 80 μmol m⁻² sec⁻¹ for 7 days (16 h/8 h L/D cycle), then transferred to 10 μmol m⁻² sec⁻¹ (10) or 50 μmol m⁻² sec⁻¹ (50) white light (16 h/8 h L/D cycle) for 5 days before seedlings were photographed. Petiole angle from the horizontal was measured for the first true leaves. Each value is the mean ± standard error (SE) of 10 seedlings.

(b) Plants were grown on soil under white light at 80 μmol m⁻² sec⁻¹ for 24 days (16 h/8 h L/D cycle.) The leaf expansion index of the 5^th rosette leaf was expressed as the ratio of the leaf area before and after artificial uncurling. Each value is the mean ± SE of 10 leaves. Images of leaf sections illustrate the leaf expansion phenotype.