Figure 1.

(A) J8-specific or (B) DT-specific serum IgG in SWISS mice (n = 5 female and n = 5 male mice) administered J8-DT/alum, DT/alum or PBS/alum with 2 or 3 intramuscular immunizations on days 0, 21 and 42 (x3 immunisations only). Mice were immunized as previously described³⁰ with minor modifications. Briefly, 4–6 week old SWISS mice (n = 5 female and n = 5 male/group housed separately) were immunized intramuscularly on day 0 with J8-DT (50 ug), DT (50 ug) or PBS formulated with alum (Alhydrogel 2%). Selected cohorts were boosted on day 21 or on days 21 and 42. Sera from the cohorts were collected on selected days pre and post-immunisation. SWISS mice were purchased from The Animal Resource Centre, Western Australia. Statistical significance was calculated using GraphPad Prism version 6. One-way Analysis of Variance (ANOVA) was applied to evaluate significant differences in absorbance levels. The Dunnett's test was performed as a post-hoc test. A P value of < 0.05 was considered significant. Data was expressed as mean ± SEM.