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. 2016 Nov 23;80(6):909–923. doi: 10.1002/ana.24815

Figure 2.

Figure 2

Phosphodiesterase 4 (PDE4) inhibitors improve motor function in Cln3Δex7/8 mice. Cln3Δex7/8 and wild‐type (WT) mice (n = 8/group) received vehicle or (A, D) rolipram (RO; 0.5 or 5mg/kg), (B, E) roflumilast (RF; 5mg/kg), or (C, F) PF‐06266047 (PF; 0.5mg/kg) beginning at 1 month of age, after which motor activity was assessed by accelerating rotarod testing at monthly intervals. Across all comparisons, significant differences between vehicle‐treated WT and Cln3Δex7/8 animals are denoted by asterisks. (A) Significant differences between Cln3Δex7/8 vehicle‐treated versus Cln3Δex7/8 5mg/kg/day rolipram are indicated by hash signs, Cln3Δex7/8 vehicle‐treated versus Cln3Δex7/8 0.5mg/kg/day rolipram are denoted by daggers, and WT vehicle‐treated versus WT 5mg/kg/day rolipram are indicated by ampersand signs. (B) Cln3Δex7/8 vehicle‐treated versus Cln3Δex7/8 roflumilast‐treated mice are indicated by hash signs, and WT vehicle‐treated versus WT roflumilast are indicated by ampersand signs. (C) Cln3Δex7/8 vehicle‐treated versus Cln3Δex7/8 PF‐06266047–treated mice are indicated by hash signs, and WT vehicle‐treated versus WT PF‐06266047–treated mice are indicated by ampersand signs. (D–F) Weights of Cln3Δex7/8 and WT mice during PDE4 inhibitor treatment. (D) Significant differences between Cln3Δex7/8 vehicle‐treated versus Cln3Δex7/8 5mg/kg/day rolipram are indicated by hash signs, Cln3Δex7/8 vehicle‐treated versus Cln3Δex7/8 0.5mg/kg/day rolipram are denoted by daggers, and WT vehicle‐treated versus WT 5mg/kg/day rolipram are indicated by ampersand signs. (E) Significant differences between WT vehicle‐treated and WT roflumilast are indicated by ampersand signs. (F) Significant differences between Cln3Δex7/8 vehicle‐treated versus Cln3Δex7/8 PF‐06266047–treated mice are indicated by hash signs, and WT vehicle‐treated versus WT PF‐06266047–treated mice are indicated by ampersand signs. For all comparisons, *, #, &, † reflect p < 0.05; **, ##, &&, †† denote p < 0.01; ***, ###, &&&, ††† reflect p < 0.001; and ****, ####, &&&& represent p < 0.0001.