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. 2016 Feb 22;28(7):975–984. doi: 10.1111/nmo.12795

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© 2016 The Authors. Neurogastroenterology & Motility Published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Intracellular recording from a myenteric neuron with Hu‐translocation. (A) Depolarization with a large current pulse failed to evoke an action potential in this neuron. (B) Intracellular injection of carboxyfluorescein confirmed the neuronal morphology. The arrow indicates the nucleus. (B' and B”) Immunohistochemical labeling revealed translocation of Hu protein to the nucleus (arrows) (scale bar = 50 μm). Images were viewed on a Nikon confocal microscope (Eclipse Ti) with 20 × objective.