Table 1.
Average weight of mushrooms harvested (g) | ||||
---|---|---|---|---|
Strain/Transformant | Flush 1 | Flush 2 | Flush 3 | Total |
A15 control | 614 | 256 | 71 | 941 |
T‐ GFP control | 534 | 188 | 24 | 746 |
S 1 | 509 | 159 | 83 | 751 |
S 3 | 502 | 299 | 133 | 934 |
S 4* | 113 | 0 | 30 | 143 |
S 5* | 137 | 50 | 55 | 242 |
AS 5* | 0 | 0 | 0 | 0 |
AS 8 | 451 | 150 | 87 | 688 |
AS 10 | 377 | 61 | 72 | 510 |
AS 13* | 147 | 0 | 29 | 176 |
ST 2 | 633 | 280 | 98 | 1012 |
ST 4* | 205 | 145 | 30 | 381 |
ST 6 | 354 | 253 | 57 | 664 |
ST 9 | 648 | 226 | 6 | 880 |
ST 11 | 644 | 195 | 71 | 909 |
Transgenic strains were grown in three replicate pots and mushrooms harvested at maturity stage 2–3 (Hammond and Nichols, 1976) over a period of 30 days. Mushrooms were collected from three successive flushes and the total number of mushrooms and weight of mushrooms recorded. Yields of Sense (S), Antisense (AS) and Sense‐Stop (ST) transformants of Spr1 were compared with two controls: non‐transformed A15 and GFP‐transformed T‐GFP. The LSD5% (Least Significant Difference) of the Total Yields was 288. The four transformants which were significantly different from the two controls are marked with an asterisk, *.