Table 1.

Mushroom fruiting results for transgenic strains.

	Average weight of mushrooms harvested (g)
Strain/Transformant	Flush 1	Flush 2	Flush 3	Total
A15 control	614	256	71	941
T‐ GFP control	534	188	24	746
S 1	509	159	83	751
S 3	502	299	133	934
S 4*	113	0	30	143
S 5*	137	50	55	242
AS 5*	0	0	0	0
AS 8	451	150	87	688
AS 10	377	61	72	510
AS 13*	147	0	29	176
ST 2	633	280	98	1012
ST 4*	205	145	30	381
ST 6	354	253	57	664
ST 9	648	226	6	880
ST 11	644	195	71	909

Transgenic strains were grown in three replicate pots and mushrooms harvested at maturity stage 2–3 (Hammond and Nichols, 1976) over a period of 30 days. Mushrooms were collected from three successive flushes and the total number of mushrooms and weight of mushrooms recorded. Yields of Sense (S), Antisense (AS) and Sense‐Stop (ST) transformants of Spr1 were compared with two controls: non‐transformed A15 and GFP‐transformed T‐GFP. The LSD5% (Least Significant Difference) of the Total Yields was 288. The four transformants which were significantly different from the two controls are marked with an asterisk, *.