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Induction of p53, p21 and Mdm2 in primary and immortal HUFs treated with Nutlin‐3a. Protein expression of p53, p21 and Mdm2 was assessed by Western blotting of whole cell lysates. Gapdh served as a loading control. (a) Primary or (b) immortal HUFs with WT TP53 (clone XN‐3N‐107) were treated for 2, 8, 24 or 48 hr ± 5 or 10 μM Nutlin‐3a. (c) Immortal HUFs with mutated TP53 were treated without (−) or with (N) 10 μM Nutlin‐3a for 24 hr.
