Table 1.

The effect of Nutlin‐3a on cell growth and p53 activity in TP53‐mutant HUF cell lines established A) prior to and B) in parallel to a Nutlin‐3a counter‐screen

	TP53 mutation status		Response of HUF cell line to Nutlin‐3a			p53 mutant function (other studies)
HUF cell line IDa	Mutation	Zygosity	Growth inhibition (5 days)	Protein induction (24 hr)	Activityb	Dominant negative
				p21			Mdm2
A) Established prior to a Nutlin‐3a counter-screen
XW‐3N‐7	Y236C	Homo‐/hemi‐	Resistant	–	–	NF	N/A
XW‐3N‐8	H179N	Homo‐/hemi‐	Resistant	–	–	PF	Yesc
XW‐3N‐9	K120M	Homo‐/hemi‐	Resistant	↑	–	NF	N/A
XW‐3N‐14	A161P R249S	Hetero‐ Hetero‐	Mixed	↑	↑	PF NF	N/A Yes3, 4
XW‐3N‐15	A161G L194R	Hetero‐ Hetero‐	Mixed	↑	↑	PF NF	Nod N/A
XN‐3N‐105	R273L	Hetero‐	Resistant	–	–	NF	Yes3, 4
XN‐3N‐110	intron 5 (SA)	Homo‐/hemi‐	Resistant	–	–	N/A	N/A
XN‐3N‐117	V157F	Hetero‐	Resistant	–	–	NF	Somec
XN‐3N‐120	V157F	Hetero‐	Resistant	–	–	NF	Somec
B) Established in parallel to a Nutlin‐3a counter‐screen
XW‐3N‐29	intron 3 (SA)	Homo‐/hemi‐	Resistant	–	–	N/A	N/A
XW‐3N‐37	R282W	Homo‐/hemi‐	Resistant	–	–	NF	Somec, nod
XW‐3N‐43	R248W	Hetero‐	Resistant	↑	–	NF	Yesc
XW‐3N‐54	H178Q H179P	Hetero‐ Hetero‐	Resistant	–	–	NF PF	N/A N/A
XW‐3N‐55	R175L	Homo‐/hemi‐	Resistant	↑	↑	PF	Noc
XW‐3N‐59	R158L	Homo‐/hemi‐	Resistant	–	–	NF	Somec
XN‐3N‐136	G245R	Homo‐/hemi‐	Resistant	–	–	NF	Yesc
XN‐3N‐137	G244A	Hetero‐	Resistant	–	–	NF	N/A
	R249T	Hetero‐				NF	N/A
XN‐3N‐140	C277F	Hetero‐	Resistant	–	–	NF	N/A
XN‐3N‐141	C277Y	Hetero‐	Sensitive; rapid recovery	↑	↑	NF	Yesc, nod
XN‐3N‐151	c124, frameshift	Homo‐/hemi‐	Resistant	–	–	N/A	N/A
XN‐3N‐156	R273S	Hetero‐	Resistant	–	–	NF	Yese
XN‐3N‐157	intron 8 (SA)	Homo‐/hemi‐	Resistant	–	–	N/A	N/A

^aXW = Xpa‐WT; XN = Xpa‐Null; 3N = 3‐NBA‐treated.

^bThe overall transactivation activity of the mutant in a yeast functional assay published by Kato et al.^d (NF = non‐functional, PF = partially functional, F = functional, N/A = not assessed).

^cDearth et al.27

^dMarutani et al.28

^eMitsumoto et al.29